Ge rpn2512e product user manual pdf download manualslib. Ge healthcare amersham ecl select western blotting detection. Amersham place little chalfont buckinghamshire, hp7 9na uk ge healthcare europe, gmbh munzinger strasse 5 d79111 freiburg germany ge healthcare biosciences corp. Fluorescence signals were detected using imagequant las 4010. Imagequant las 4000 data file ge healthcare life sciences.
Typhoon fla 9000 quick start guide baylor university. If you find that there are any issues with the pumps performance, however, it is returnable for a full refund within 30 days. Ge imagequant las4000 fluorescence image analyzer box amersham imager 600 unit4 here is a ge imagequant las4000 biomolecular fluorescence image analyzer light box pred amersham imager 600 unit4this system is set up for the following studieschemilumine. Content western blotting handbook and troubleshooting guide publish date. The clarity ecl has been super pricey but we have been fortunate to find alternative cl substrates that were cheaper and still work for highly sensitive detection of our western blots. Box chemi xx6gel documentation and ecl detection syngene is the most popular gel documentation system in new england. Western blotting using enhanced chemiluminescence thermo.
The uv and white light transilluminators and optional epifluorescent light sources are housed inside the cabinet. Sure, in gel fluorescence figure 7 and media plates figure 8 are not much of a stretch, but its the azure imaging systems unmatched depthoffield that enables imaging more threedimensional samples such as mice figure 9 and zebrafish figure 10. Amersham imager 680 combined with amersham ecl plex provides highquality data in applications that demand high sensitivity over a wide dynamic range. Amersham ecl select western blotting detection reagent from. Please be aware the import taxes may char ge d by your post office are buyers responsibility to pay. Imagequant las 4000 mini biomolecular imager from ge healthcare.
Multiple options for faster western blotting biocompare. Amersham ecl mouse igg hrplinked whole antibody from sheep. A selection of a dilution series is shown in the image. Amersham ecl western blotting detection reagents and. Amersham ecl prime blocking agent amersham ecl blocking agent bsa blocking agent nonfat dry milk 6. Amersham ecl gel box 89237902 amersham ecl gel, 10% pack of 10 gels 89237862 amersham ecl gel, 420% pack of 10 gels 89237870. Imagequant las 4010 4000 4000 mini quick start guide. Data file 29098107 ac imaging systems, software, and. All users must read this entire manual to fully understand the safe use of typhoono trio.
Quantitation of signal intensity was performed using genesnap image acquisition software. Furthermore, the minimal crosstalk of amersham imager 680, and the spectrally resolved dyes cy2, cy3, and cy5, makes it a suitable system for a wide. Box 27 piscataway, nj 0885527 usa ge healthcare japan corporation sanken building 3251 hyakunincho, shinjukuku, tokyo 1690073 japan. Amersham ecl rainbow marker full range full range, cytiva. Amersham imager 600 operating instructions 29064517 ad. Amersham ecl gels cytiva, formerly ge healthcare life. Amersham ecl gels cytiva, formerly ge healthcare life sciences. Data file 28961074 ac imaging systems, software, and. A license to use the cy and cydye trademarks for commercial purposes is.
Amersham ecl select detection reagent is manufactured in compliance with our iso 9001 certified quality management. High signal intensity and sensitivity allows the use of highly diluted primary and secondary. Which is the best and more sensitive western blot imaging. The core of the system is amersham ecl gel, a precast gel available in a variety of concentrations and well configurations. Ge healthcare amersham ecl gel box a horizontal unit for high quality protein electrophoresis. Selection guide amersham ecl western blotting detection. Horizontal amersham ecl gel system from ge healthcare life. Amersham ecl gel and gel box user manual 28994333 ac. Amersham pharmacia biotech inc 800 centennial avenue, po box 27, piscataway, nj 08855 usa amersham pharmacia biotech europe gmbh munzinger strasse 9, d79111 freiburg, germany amersham pharmacia biotech kk, sanken bldg. Amersham ecl rainbow marker full range has been used as a molecular weight marker for immunoblotting and sodium dodecyl sulfate polyacrylamide gel electrophoresis sdspage.
Ge healthcare launches the horizontal amershamtm ecltm. The ecl box system uses only 200 ml of the running buffer. Preparing amersham ecl gel box recommendations power supply recommended power supply is eps 301. Amersham ecl prime is a highly sensitive chemiluminescent detection reagent and is characterized by extremely stable signal emission. Data file 28961074 ab imaging systems, software, and. Imagequant las 4000 mini biomolecular imager from ge. This product or portions thereof is manufactured under an exclusive license from carnegie mellon university under us patent number 5,268,486 and equivalent patents in the us and other countries. Improved performance of amersham ecl prime compared with other chemiluminescent reagents is made possible due to a highperformance enhancer and a catalyst increasing signal intensity, duration, and sensitivity.
Imaging systems, software, and accessories amersham typhoon. Amersham, ecl, ecl plus, hybond, hypercassette, hyperfilm. Amersham ecl start western blotting detection reagent. Features and benefits monitor progress of protein electrophoresis and assess transfer efficiency and molecular weight of blotted proteins. Cy3 dige fluor, ecl plex cy3 cy5, alexa fluor 633, toto 3, did, cy5 dige fluor, ecl plex cy5 carbonic anhydrase in lmw marker amersham ecl gel 420% sdspage trisglycine excitation. Hrpconjugated antibodies optimized for use with amersham ecl. Amersham ecl gel box cytiva, formerly ge healthcare life. May 30, 20 protocol overview add 90 ml of running buffer each to the anode and cathode chamber of the ecl gel box, remove the amersham gel cassette various % gels available form the pouch and rinse with distilled water, remove the tapes form the two legs of the cassette. The uv and white light transilluminators and optional epifluorescent light sources are housed inside. The gel is compatible with standard laemmli buffers, and has a shelf life of up to 12 months with no compromise in performance over time. Amersham pharmacia biotech europe gmbh munzinger strasse 9, d79111 freiburg. Box ichemixt imager syngene at the indicated times.
Ge 0004696 grminiv2 healthcare amersham ecl gel 28990603. Protein analysis amersham ecl start western blotting. Amersham biosciences inc 800 centennial avenue po box 27 piscataway nj08855 usa amersham biosciences europe gmbh munzinger strasse 9 d79111 freiburg germanyrpn2124psab. Ge imagequant las4000 biomolecular fluorescence image. The special formulation of pierce ecl substrate makes it the ideal substitute for amersham ecl substrate without the need for additional optimization of assay conditions.
Since the introduction of the first enhanced chemiluminescent ecl detection reagent for western blotting amersham ecl in 1990, the portfolio of products offered by cytiva has been improved and optimized across all western blotting requirements from electrophoresis and transfer equipment to highly sensitive detection systems and software. Amersham biosciences uk limited amersham place little chalfont buckinghamshire england hp7 9na amersham biosciences ab se751 84 uppsala sweden amersham biosciences corp 800 centennial avenue po box 27 piscataway nj. Amersham ecl plex western blotting system, in combination with typhoon. Po box 27 piscataway, nj 08855 usa amersham pharmacia biotech europe gmbh postfach 5480 d79021 freiburg. Amersham imager 600 operating instructions 29064517 ac. Amersham ecl gel box from ge healthcare life sciences. Amersham ecl prime western blotting detection reagent deliver. View the latest ge healthcare announcements and thought leadership articles to learn more about how we are helping elevate healthcare. Amersham ecl prime blocking agent 2% in pbst primary antibody.
Amersham ecl plex cy2 and cy3 conjugated secondary antibodies. By doing this, we are excluding manual extra steps. Please read this entire manual to fully understand the safe and effective use of this product. Amersham ecl select detection reagent is compatible with pvdf and nitrocellulose membranes such as amersham hybond and amersham protran product range, amersham ecl prime blocking agent, amersham ecl blocking agent and other commonly used blocking agents such as bsa and nonfat dry milk. High signal intensity makes amersham ecl select suitable for the most demanding western blotting applications, allowing for detection of minute protein quantities. You can run up to 26 samples or accommodate 11 cm readystrip ipg strips. The troubleshooting guide below addresses problems common to the. If raw data, high sensitivity, image resolution, an affordable price, and great service are important factors in making your decision for a gel imaging system, contact us or call 6172864632. Amersham ecl gel and amersham ecl gel box constitute a horizontal mini gel system for highquality protein electrophoresis. The products in this guide are available from ge healthcare distributors. Typhoon trio is intended for laboratory use only, not for clinical or in vitro use, or for diagnostic purposes. Selection guide amersham ecl western blotting detection reagents. Ge amersham imager 600 operating instructions manual pdf. Ge healthcare amersham ecl gel box horizontal format.
This may be achieved manually by dounce homogenization or mechanically by potter. High signal intensity makes amersham ecl select suitable for the most demanding western blotting. Its long lasting signals allow for multiple exposures and a convenient time window. A copy of these terms and conditions is available on request. A twofold dilution series of nih3t3 cell lysate starting at 5 g total protein was subjected to chemiluminescent western blotting and erk was detected with amersham ecl select. It is easy to use the system, and the gel box does not require any assembly. Amersham ecl prime western blotting detection reagent. Tubulin and erk12 were targeted in a dilution series of cho cell lysate by using mouse antitubulin and rabbit antimap kinase erk 12 primary antibodies. See ecl gel box user manual 28994333 for more information on power supply compatibility. Gels are processed using amersham ecl gel box, a dedicated electrophoresis unit with horizontal design, and significantly increased ease of use compared to vertical systems. High intensity of the emitted signal means that amersham ecl prime is suitable for working with low abundance proteins long lasting, stable signal 3 hours. The criterion cell can run one or two criterion gels, which are wider and longer than traditional mini gels. Amersham ecl select western blotting detection reagent with highly intense signal output, for detection of medium to very low protein levels.
For more information on manual settings see set exposure time, on page high dynamic range checkbox. Because standard protocols require multiple manual manipulations, the elimination of any of these steps makes the work flow shorter and less cumbersome. Ecl plus contains lumigen ps3 substrate and is sold under exclusive license from lumigen inc. Typhoon fla 9500 biomolecular imager ge healthcare life. Data file 28961072 ab imaging systems, software, and. Amersham ecl select is a high sensitivity reagent for chemiluminescent western blotting detection.
Multiplex detection of endogenous proteins by amersham ecl plex western blotting. Important information if it is not shown in the picture then it probably does not come with the item usually power cord and connector cords are not included please email me if you have a question before purchasing. Use the check box for image capture with high dynamic range. This user documentation contains cautions concerning the safe use of. For those familiar with amersham ecl plus, amersham ecl prime is the replacement product that offers the same sensitivity, stability. Percoll methodology and applications 18111569 ficollpaque plus. The gel was imaged with amersham typhoon using normal scan speed. Data file 29020166 ab imaging systems, software, and. Horizontal amersham ecl gel system from ge healthcare.
Place the cassette in the ecl gel box, with wellside in the cathode side. Ge healthcare amersham ecl ge l box unit is untested and functionality unknown. Amersham protran are nitrocellulose membranes compatible with all chemiluminescent western blotting substrates. Ge healthcare life sciences has launched the amersham ecl gel electrophoresis system with precast gels and easytouse ccd imagers. Amersham ecl prime and amersham ecl select reach a limit of detection in the picogram range with imagequant las 500 and a dynamic range covering three orders of magnitude. Furthermore, the minimal crosstalk of amersham imager 680, and the spectrally resolved dyes cy2, cy3. The system can house up to four lasers simultaneously, from a choice of five laser excitation wavelengths 473, 532, 635, 685, and 785 nm. Recommended gel and western blot documentation system. If you want to capture ecl you need a good cooled ccd camera the light box should have a good quality uv box, internal light source and filters on the camera to get different fluorescent probes. Amersham ecl select western blotting detection reagent. Ge 18608301 p950 amersham pharmacia biotech sample pump. Amersham ecl prime or portions thereof is manufactured and sold under license from cyanagen srl and is subject of us patent 7,855,287, us patent 7,803,573, and italian application number to2010a000580, together with other equivalent granted patents and patent.
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